Enhanced Biofilm Formation of Streptococcus gordonii with Lipoprotein Deficiency

In this study, we investigated the role of lipoproteins ofS. gordonii in the bacterial biofilm formation using its lipoprotein ‐deficient strain (Δlgt). TheS. gordonii Δlgt exhibited increased biofilm formation on the human dentin slices or on the polystyrene surfaces compared to the wild ‐type strain, while its growth rate did not differ from that of the wild‐type. In addition, theS. gordonii Δlgt strain exhibited the enhanced mRNA expression of LuxS and AI ‐2 production, which is known to be a positive regulator of biofilm formation, compared to the wild‐type. Concordantly, the augmented biofilm formation ofS. gordonii Δlgt was attenuated by an AI ‐2 inhibitor,D‐ribose. In addition, the purified lipoproteins ofS. gordonii efficiently inhibited the biofilms ofS. gordonii and itsΔlgt. Treatment with lipoproteins from purifiedS. gordonii inhibited the biofilm formation ofS. gordonii wild ‐type andΔlgt. Taken together, these results suggest that lipoprotein ‐deficientS. gordonii form biofilms more effectively than the wild ‐type strain, which might be related with the AI‐2 quorum‐sensing system.
Source: Molecular Oral Microbiology - Category: Microbiology Authors: Tags: ORIGINAL ARTICLE Source Type: research