Development of a spermatogonia cryopreservation protocol for blue catfish, Ictalurus furcatus.

Development of a spermatogonia cryopreservation protocol for blue catfish, Ictalurus furcatus. Cryobiology. 2020 Oct 12;: Authors: Abualreesh M, Myers JN, Gurbatow J, Johnson A, Xing, Wang J, Li S, Coogan M, Vo K, El Husseini N, Dunham RA, Butts IAE Abstract Sustainability of channel catfish, Ictalurus punctatus ♀ × blue catfish, Ictalurus furcatus ♂ hybrid aquaculture relies on new innovative technologies to maximize fry output. Transplanting spermatogonia from blue catfish into channel catfish hosts has the potential to greatly increase gamete availability, and cryopreservation would make these cells readily accessible year-round. However, a protocol for cryopreserving blue catfish testicular tissues has not yet been fully developed. Therefore, the objectives of this experiment were to identify the best permeating [dimethyl sulfoxide (DMSO), ethylene glycol (EG), glycerol, methanol] and non-permeating (lactose or trehalose with egg yolk or BSA) cryoprotectants, their optimal concentrations, and the best freezing rate (-0.5, -1.0, -5.0, -10 °C/min until -80 °C) that yields the highest number of live type A spermatogonia cells and highest viability percentages after cryopreservation. Results showed that all of these factors had significant impacts on post-thaw cell production and viability. DMSO was the most efficient permeating cryoprotectant at a concentration of 1.0 M. The optimal concentration of each cryoprotect...
Source: Cryobiology - Category: Biology Authors: Tags: Cryobiology Source Type: research