Platinum nanozyme-encapsulated poly(amidoamine) dendrimer for voltammetric immunoassay of pro-gastrin-releasing peptide.

Platinum nanozyme-encapsulated poly(amidoamine) dendrimer for voltammetric immunoassay of pro-gastrin-releasing peptide.
Anal Chim Acta. 2020 Oct 16;1134:106-114
Authors: Zhi LJ, Sun AL
Abstract
An innovative electrochemical immunosensing platform was designed for the sensitive monitoring of lung cancer biomarker (pro-gastrin-releasing peptide; ProGRP) by using platinum nanoparticles encapsulated inside dendrimers (PtDEN) as enzymatic mimics for the signal amplification. PtDEN nanocomposites were prepared through a simple chemical reduction method with the assistance of NaBH4. Thereafter, PtDEN-labeled anti-ProGRP secondary antibody was launched for the detection of target analyte with a sandwich-type assay format on anti-ProGRP capture antibody-modified screen-printed carbon electrode. Accompanying formation of immunocomplex, the labeled PtDENs electrochemically oxidized 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide to produce a well-defined voltammetric signal within the applied potentials. Thanks to the high-efficient catalytic efficiency of platinum nanoparticles and high-loading ability of dendrimer, improved analytical features were acquired with PtDENs relative to platinum nanoparticles alone. Using PtDENs labeling strategy, the properties and factors influencing the analytical performance of electrochemical immunosensor were studied in detail. The strong bioconjugation of antibodies with the PtDENs...

Source: Analytica Chimica Acta - October 16, 2020 Category: Chemistry Authors: Zhi LJ, Sun AL Tags: Anal Chim Acta Source Type: research