Anti-dsDNA blockade ameliorates systemic lupus erythematosus in MRL/Faslpr mice through ameliorating inflammation via PKC δ-NLRC4 axis.

Anti-dsDNA blockade ameliorates systemic lupus erythematosus in MRL/Faslpr mice through ameliorating inflammation via PKCδ-NLRC4 axis. Biochem Cell Biol. 2020 Oct 16;: Authors: Yang F, Yang Y, Zeng W Abstract Anti-double-stranded DNA (anti-dsDNA) has been identified to be closely related to brain inflammatory burden after ischemic stroke. Here, we studied the inflammatory cascade after dsDNA and investigated the mechanisms of its pro-inflammatory role in systemic lupus erythematosus (SLE). The IL-1β and IL-6 levels in serum of SLE patients and controls were evaluate by ELISA, and the caspase-1 expression was detected using RT-qPCR. IL-1β and IL-6 were increased in serum of SLE patients. Caspase-1 expression was promoted and positively correlated with pro-inflammatory factor levels, and anti-dsDNA was also elevated and positively related with the mean fluorescent intensity (MFI) of caspase-1. Additionally, MRL/Faslpr mice were used for detecting the functions of PRKCD encoding protein kinase c delta (PKCδ) and NLRC4 in vivo. In MRL/Faslpr mice, the renal injury was aggravated, and the levels of pro-inflammatory factors were increased. Increased NLRC4 in mice exacerbated renal injury and increased levels of pro-inflammatory factors, while inhibition of PKCδ contributed to opposite trends. These findings provide unique perspectives on pathogenesis of SLE and indicate that inhibition of anti-dsDNA could attenuate renal inflammatory ...
Source: Biochemistry and Cell Biology - Category: Biochemistry Authors: Tags: Biochem Cell Biol Source Type: research