Ram semen quality can be assessed by flow cytometry several hours after post-fixation.

In conclusion, several tested markers were able to withstand formaldehyde fixation of ram semen samples as follows: annexin V and FLICA for apoptosis; PNA for acrosomal status; MitoTracker Red CMXRos for mitochondrial activity; and CellROX Green for oxidative status in combination with a suitable live/dead fixable viability dye. This optimized methodology could help to comprehensively analyse the quality of ram semen from local farms countrywide.
PMID: 33046151 [PubMed - as supplied by publisher]

Source: Zygote - October 13, 2020 Category: Reproduction Medicine Authors: Vašíček J, Svoradová A, Baláži A, Jurčík R, Macháč M, Chrenek P Tags: Zygote Source Type: research