Silencing of long non ‐coding RNA DLX6‐AS1 weakens neuroblastoma progression by the miR‐513c‐5p/PLK4 axis

AbstractEmerging evidence has demonstrated the crucial roles of long noncoding RNAs in human cancers, including neuroblastoma (NB). DLX6 antisense RNA 1 (DLX6 ‐AS1) has been identified as an oncogenic driver in NB. However, the mechanisms of DLX6‐AS1 in NB progression are not fully understood. Our data showed that DLX6‐AS1 was significantly overexpressed in NB tissues and cells. Moreover, DLX6‐AS1 silencing repressed NB cell viability, colony form ation, migration, and invasion, and promoted cell cycle arrest and apoptosis in vitro, as well as decreased tumor growth in vivo. Mechanistically, DLX6‐AS1 operated as a miR‐513c‐5p sponge. MiR‐513c‐5p mediated the regulation of DLX6‐AS1 on NB cell malignant progression in vitro. PLK4 wa s a target of miR‐513c‐5p‐ and DLX6‐AS1‐controlled PLK4 expression via sponging miR‐513c‐5p. Furthermore, the suppressive effect of miR‐513c‐5p overexpression on NB cell malignant progression in vitro was reversed by PLK4 upregulation. Our findings identified a novel regulatory mec hanism, the DLX6‐AS1/miR‐513c‐5p/PLK4 axis, in NB progression, highlighting a strong rationale for developing DLX6‐AS1 as a new target for NB management.

Source: IUBMB Life - October 8, 2020 Category: Research Authors: Peisheng Jia, Erhu Wei, Huiqiong Liu, Tingting Wu, Huaili Wang Tags: RESEARCH COMMUNICATION Source Type: research