Exploring the role of E coli derived enzyme, oxyrase, as an oxygen scavanger to improve the cryotolerance of spermatozoa of sahiwal bull.

Exploring the role of E coli derived enzyme, oxyrase, as an oxygen scavanger to improve the cryotolerance of spermatozoa of sahiwal bull. Cryobiology. 2020 Oct 07;: Authors: Ngou AA, Ghosh SK, Prasad JK, Katiyar R, Kumar A, Rautela R, Amarjeet, Srivastava N, Kumar A Abstract The current study intended to optimize the concentration of Oxyrase in the semen dilutor and to evaluate its effect on freezability of spermatozoa of Sahiwal bulls. Supplementation of Oxyrase at 0.125 IU/mL concentration significantly reduced dissolved oxygen (DO) in the dilutor to 4 ppm in 16-18 min at 35 °C. For supplementaion studies, a total of 24 eajaculates were categorized into poor and good ejaculates categories (n = 12 each) based on their initial progressive motility. Each ejaculate was further divided into two aliquotes. The first aliquote was diluted with tris-egg yolk extender without Oxyrase (control group) whereas, in the treatment group, Oxyrase was supplemented at the concentration of 0.125 IU/mL of extender. The parameters evaluated include seminal plasma cholesterol (SPC) and plasma membrane phospholipids (PMP) at fresh, while IPM, acrosomal and plasma membrane integrity, SPC, PMP and oxidative stress parameters like lipid peoxidation (LPO), total antioxidant capacity (TAC) and reactive ooxygen species (ROS) were evaluated at pre-freeze and post-thaw stages. The IPM and acrosomal intactness was higher (p < 0.05) in treatment g...
Source: Cryobiology - Category: Biology Authors: Tags: Cryobiology Source Type: research