Establishment and characterization of a continuous cell line from heart of Nile tilapia Oreochromis niloticus and its susceptibility to Tilapia lake virus.

Establishment and characterization of a continuous cell line from heart of Nile tilapia Oreochromis niloticus and its susceptibility to Tilapia lake virus. J Virol Methods. 2020 Oct 06;:113989 Authors: Yadav MK, Rastogi A, Criollo Jaoquin MP, Verma DK, Rathore G, Swaminathan TR, Paria A, Pradhan PK, Sood N Abstract In the present study, we have developed a continuous cell line from the heart tissue of the Oreochromis niloticus and used for studying susceptibility to Tilapia Lake Virus (TiLV). The cell line, designated as OnH, has been subcultured for more than 82 passages. The optimal growth of OnH cells was observed at 28- 32 °C L-15 medium supplemented with 20% fetal bovine serum. Karyotype analysis revealed that the modal chromosome number of OnH cells was 44. Partial amplification and sequencing of 16S rRNA gene confirmed the origin of OnH cell line from O. niloticus. Immunophenotyping revealed that OnH cells were of epithelial origin. These cells were successfully transfected with pAcGFP1-N1 mammalian expression vector. OnH cells showed cytopathic effects following inoculation with TiLV. The virus titration study indicated that the cells were highly susceptible to TiLV with TCID50 value of 105.3/ml. The qRT-PCR studies revealed that the optimal temperature for TiLV replication in OnH cells was 28 °C. Further, transmission electron microscopy of TiLV-infected OnH cells showed a number of electron-dense virus particles mea...
Source: Journal of Virological Methods - Category: Virology Authors: Tags: J Virol Methods Source Type: research