Towards a Continuous Manufacturing Process of Protein-Loaded Polymeric Nanoparticle Powders.

Towards a Continuous Manufacturing Process of Protein-Loaded Polymeric Nanoparticle Powders. AAPS PharmSciTech. 2020 Oct 06;21(7):269 Authors: Schiller S, Hanefeld A, Schneider M, Lehr CM Abstract To develop a scalable and efficient process suitable for the continuous manufacturing of poly(lactic-co-glycolic acid) (PLGA) nanoparticles containing ovalbumin as the model protein. PLGA nanoparticles were prepared using a double emulsification spray-drying method. Emulsions were prepared using a focused ultrasound transducer equipped with a flow cell. Either poly(vinyl alcohol) (PVA) or poloxamer 407 (P-407) was used as a stabilizer. Aliquots of the emulsions were blended with different matrix excipients and spray dried, and the yield and size of the resuspended nanoparticles was determined and compared against solvent displacement. Nanoparticle sizes of spray-dried PLGA/PVA emulsions were independent of the matrix excipient and comparable with sizes from the solvent displacement method. The yield of the resuspended nanoparticles was highest for emulsions containing trehalose and leucine (79%). Spray drying of PLGA/P-407 emulsions led to agglomerated nanoparticles independent of the matrix excipient. PLGA/P-407 nanoparticles pre-formed by solvent displacement could be spray dried with limited agglomeration when PVA was added as an additional stabilizer. A comparably high and economically interesting nanoparticle yield could be achieved with a process suitable...
Source: AAPS PharmSciTech - Category: Drugs & Pharmacology Authors: Tags: AAPS PharmSciTech Source Type: research

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Source: Annals of Hepatology - Category: Gastroenterology Tags: Ann Hepatol Source Type: research
Conditions:   NAFLD;   NASH - Nonalcoholic Steatohepatitis Intervention:   Diagnostic Test: Liver Incyte Sponsors:   Indiana University;   Sonic Incytes Recruiting
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Source: Free Radical Research - Category: Research Tags: Free Radic Res Source Type: research
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Source: Cancers - Category: Cancer & Oncology Authors: Tags: Review Source Type: research
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Source: Yonsei Medical Journal - Category: Universities & Medical Training Authors: Tags: Yonsei Med J Source Type: research
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Source: World Journal of Gastroenterology : WJG - Category: Gastroenterology Authors: Tags: World J Gastroenterol Source Type: research
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