GSE138616 Ribosome Profiling Reveals MK-801-Induced Differential Translation in the Prefrontal Cortex of Acute MK-801-Exposed Male Mice

Contributors : Haihong Ye ; Jialu ZhaoSeries Type : Expression profiling by high throughput sequencing ; OtherOrganism : Mus musculusDifferential translation significantly contributes to gene expression regulation and FMRP is one of the upstream translation regulators in the PFC of MK-801-exposed juvenile male miceAcute exposure to the NMDA receptor antagonist MK-801 induces schizophrenia-like behavioral endophenotypes in juvenile male mice. However, the genome-wide gene expression changes that occur at the translation level in the brains of this model are unclear. Here, we performed ribosome profiling analysis on the prefrontal cortices of acute MK-801-exposed juvenile male mice. Changes in translation efficiency (TE) alone affect expression of 28.7% of genes. Only 14.1% genes were affected at only the mRNA level, and the majority of transcriptional changes were buffered at the level of translation. Genes with up-regulated TE were enriched in neural development and synapse-related gene ontologies. We observed a significantly greater number of Fragile X Mental Retardation Protein (FMRP) target genes in the TE up-regulated gene set (28.4%) than in the down-regulated gene set (15.3%). Consistently, FMRP protein was degraded in the prefrontal cortex of MK-801-exposed mice. Our findings indicate that differential translation significantly contributes to gene expression regulation, and FMRP functions as one of the upstream translation regulators in the prefrontal cortex of MK-80...

Source: GEO: Gene Expression Omnibus - October 9, 2020 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Other Mus musculus Source Type: research