Glioma ‐induced peritumoral hyperexcitability in a pediatric glioma model

The effect of pediatric glioma cells on the function of surrounding neurons during early development is unknown. We intrancranially injected patient ‐derived pediatric glioma cells in young pups and examined on the function of peritumoral neurons. Pediatric glioma cells induced changes in the intrinsic properties of periturmoral neurons, lowered the threshold of hyperexcitability and exhibited an early onset of depolarization block. AbstractEpileptic seizures are among the most common presenting symptom in patients with glioma. The etiology of glioma ‐related seizures is complex and not completely understood. Studies using adult glioma patient tissue and adult glioma mouse models, show that neurons adjacent to the tumor mass, peritumoral neurons, are hyperexcitable and contribute to seizures. Although it is established that there are phenotypi c and genotypic distinctions in gliomas from adult and pediatric patients, it is unknown whether these established differences in pediatric glioma biology and the microenvironment in which these glioma cells harbor, the developing brain, differentially impacts surrounding neurons. In the present stu dy, we examine the effect of patient‐derived pediatric glioma cells on the function of peritumoral neurons using two pediatric glioma models. Pediatric glioma cells were intracranially injected into the cerebrum of postnatal days 2 and 3 (p2/3) mouse pups for 7 days. Electrophysiological recordin gs showed that cortical layer 2/3 peri...

Source: Physiological Reports - October 7, 2020 Category: Physiology Authors: Lata Chaunsali, Bhanu P. Tewari, Allison Gallucci, Emily G. Thompson, Andrew Savoia, Noah Feld, Susan L. Campbell Tags: ORIGINAL RESEARCH Source Type: research