Characterization of medical relevant anaerobic microorganisms by isothermal microcalorimetry.

Characterization of medical relevant anaerobic microorganisms by isothermal microcalorimetry. Anaerobe. 2020 Oct 02;:102282 Authors: Corvec S, Seiler E, Wang L, Moreno MG, Trampuz A Abstract Detection of anaerobe bacteria by culture methods requires appropriate media, special growth conditions, additional detection techniques and it typically takes several days. Therefore, anaerobes are often missed in patient specimens under routine culture conditions. Microcalorimetry may provide a simple and accurate real-time method for faster and better detection of anaerobes. An isothermal calorimeter was used for calorimetric experiments, which detect minimal changes of temperature over time. In order to find optimal growth conditions, seven reference or clinical strains of medical relevant anaerobe bacteria were tested under different circumstances. First, the strains were tested with different growth media. After determining the optimal medium for each strain, the gas phase was modified by adding 3 mL or 4 mL medium, to evaluate growth under conditions with less oxygen. Cooked Meat Medium was best supporting growth of the tested strains, including Cutibacterium acnes, Fusobacterium nucleatum, Finegoldia magna, Parvimonas micra, Bacteroides fragilis and Actinomyces odontolyticus, followed by thioglycolate. The best medium to detect Clostridioides difficile was H-Medium. All tested strains showed better growth in 4 mL medium than in 3 ...
Source: Anaerobe - Category: Microbiology Authors: Tags: Anaerobe Source Type: research