Molecular characterization of clinical isolates from vascular access infection: A single ‐institution study

In this study, we investigated the molecular profiles of different bacterial isolates retrieved from various types of VA grafts. We collected clinical isolates from hemodialysis patients with VAIs in our institution for the period between 2013 and 2018. We identified the bacterial isolates using standard biochemical procedures; we used a polymerase chain reaction for coagulase‐nega tive staphylococci (CoNS) andBurkholderia cepacia complex (BCC) species identification. The antibiotic resistance and molecular profile were analyzed using the disk diffusion method and multilocus sequence typing, respectively. We studied 150 isolates retrieved from patients with VAI and observed thatStaphylococcus aureus was the predominant bacterial species, followed byS. argenteus, BCC, and CoNS. According to multilocus sequence typing data, we identified a wide variety of sequence types (STs) inS. aureus isolates, with ST59, ST45, and ST239 being the predominant types.Burkholderia cepacia with two new ST types, namely ST1723 and ST1724, accounted for most of the BCC infections, along with ST102B. contaminans, which were mainly isolated from infected tunneled ‐cuffed catheters. In summary, the increased incidence ofS. argenteus and BCC infections provides insights into their potential clinical effects in VAIs. The various STs identified in different bacterial species indicate the high genetic diversity of bacterial species isolated from VAIs in our institution.
Source: MicrobiologyOpen - Category: Microbiology Authors: Tags: Original Article Source Type: research