Comparative thermo-stability of two Rift Valley fever virus vaccine candidate CL13T with a recombinant arMP-12 ΔNSm21/384.

Comparative thermo-stability of two Rift Valley fever virus vaccine candidate CL13T with a recombinant arMP-12ΔNSm21/384. Bioinformation. 2020;16(7):547-554 Authors: Daouam S, Boumart Z, Elarkam A, Hamdi J, Tadlaoui KO, Ennaji MM, Harraka M Abstract Rift Valley fever (RVF) is a zoonotic, viral disease, transmitted by mosquitoes, characterized by high mortality rates in young animals. RVF is an endemic and enzootic disease in the Arabian Peninsula and Africa, causing public health and economic instability. Therefore, it is important to develop vaccines to minimize outbreaks and combat the disease. We documented the stability of the thermo-stability of live attenuated RVF CL13T and recombinant arMP-12ΔNSm21/384 vaccine candidates at different temperatures, including these vaccine viruses in liquid and lyophilized form. The study revealed that both CL13T and recombinant arMP-12ΔNSm21/384 strains were stable for more than 18 months at 4°C. We show that at room temperatures (37°C and 45°C) the CL13T was less temperature sensitive than MP-12NSm-del in both lyophilized and liquid form. These findings are useful for the preparation of RVF vaccines that will avoid the need for a cold chain and therefore, will improve the application of the vaccines under field conditions. PMID: 32994680 [PubMed]
Source: Bioinformation - Category: Bioinformatics Authors: Tags: Bioinformation Source Type: research

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Conclusions/SignificanceThe high seroprevalence in all age groups and evidence of year-round viral circulation provide evidence for a hyperendemic situation in the study area. This is the first study to directly estimate infection rate of RVFV in livestock in an endemic area in the absence of reported outbreaks and provides the basis for further investigation of factors affecting viral circulation and mechanisms for virus survival during interepidemic periods.
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
In this study, we exposed apically well-differentiated human NECs cultured at the ALI to the related flaviviruses ZIKV, JEV, WNV, and Usutu virus (USUV). We selected these viruses due to the recent increasing evidences of potential threat to humans (Cadar et al., 2017; Simonin et al., 2018). We show that NECs are particularly susceptible to JEV and WNV infection and to other flaviviruses included in this study. Infection with each virus led to shedding of infectious virus particles through the apical and basolateral surfaces and triggered host mechanisms at the level of inflammatory and antiviral mediators. Given...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Abstract Rift Valley fever virus (RVFV) is an important mosquito-borne pathogen with devastating impacts on agriculture and public health. With outbreaks being reported beyond the continent of Africa to the Middle East, there is great concern that RVFV will continue to spread to non-endemic areas such as the Americas and Europe. There is a need for safe and high throughput serological assays for rapid detection of RVFV during outbreaks and for surveillance. We evaluated a multiplexing fluorescence microsphere immunoassay (FMIA) for the detection of IgG and IgM antibodies in ruminant sera against the RVFV nucleocap...
Source: Journal of Virological Methods - Category: Virology Authors: Tags: J Virol Methods Source Type: research
elmy Rift Valley fever (RVF) is an emerging transboundary, mosquito-borne, zoonotic viral disease caused high morbidity and mortality in both human and ruminant populations. It is considered an important threat to both agriculture and public health in African and the Middle Eastern countries including Egypt. Five major RVF epidemics have been reported in Egypt (1977, 1993, 1994, 1997, and 2003). The virus is transmitted in Egypt by different mosquito's genera such as Aedes, Culex, Anopheles, and Mansonia, leading to abortions in susceptible animal hosts especially sheep, goat, cattle, and buffaloes. Recurrent RVF ...
Source: Viruses - Category: Virology Authors: Tags: Review Source Type: research
Publication date: April 2018Source: Current Opinion in Virology, Volume 29Author(s): Baptiste Dungu, Baratang A Lubisi, Tetsuro IkegamiRift Valley fever (RVF) is a zoonotic mosquito-borne bunyaviral disease associated with high abortion rates, neonatal deaths, and fetal malformations in ruminants, and mild to severe disease in humans. Outbreaks of RVF cause huge economic losses and public health impacts in endemic countries in Africa and the Arabian Peninsula. A proper vaccination strategy is important for preventing or minimizing outbreaks. Vaccination against RVF is not practiced in many countries, however, due to absenc...
Source: Current Opinion in Virology - Category: Virology Source Type: research
In this study, we aimed to strengthen the attenuation profile of the MP-12 vaccine strain via the introduction of 584 silent mutations. To minimize the impact on protective efficacy, codon usage and codon pair bias were not de-optimized. The resulting rMP12-GM50 strain showed 100% protective efficacy with a single intramuscular dose, raising a 1:853 mean titer of plaque reduction neutralization test. Moreover, outbred mice infected with one of three pathogenic reassortant ZH501 strains, which encoded rMP12-GM50 L-, M-, or S-segments, showed 90%, 50%, or 30% survival, respectively. These results indicate that attenuation of...
Source: Vaccine - Category: Allergy & Immunology Authors: Tags: Vaccine Source Type: research
RIFT Valley fever virus (RVFV) is a mosquito-borne virus, member of the genus Phlebovirus (family Bunyaviridae), which affects mainly ruminant species. It may cause severe economic losses through abortions and high mortality among newborns, and through the heavy control costs and the trade restrictions imposed. Rift Valley fever (RVF) is also a zoonotic disease with potentially severe consequences for infected people, including haemorrhagic fever, meningoencephalitis, renal failure, blindness and, in some cases, death. RVFV is transmitted through the bites of various species of mosquitoes (typically the Aedes or Culex gene...
Source: Veterinary Record - Category: Veterinary Research Authors: Tags: Research Source Type: research
Abstract: The viruses that cause the Viral Haemorrhagic Fevers (VHFs) are largely zoonotic. Consequently, the endemic areas for the various VHFs are limited to the distribution of their mammalian reservoirs and/or arthropod vectors. Ecological changes, agricultural and cultural practices, population migration and land use may have an affect on the emergence and occurrence of the VHfs . Consequently a ‘One Health’ approach is important in surveillance, response and control of outbreaks of Filoviruses (Marburg and Ebola), the Bunyaviridiae (Crimean Congo haemorraghic fever and Rift Valley fever) and Flaviviruses ...
Source: International Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Type: Invited Presentation Source Type: research
In this study, a serological surveillance was conducted to detect antibodies against RVFV. A total of 2382 serum samples from goats and cattle were randomly collected from nine areas in South Korea from 2011 to 2013. These samples were tested for antibodies against RVFV, using commercial ELISA kits. None of the goats and cattle were positive for antibodies against RVFV. This finding suggests that this disease is not present in South Korea, and furthermore presents the evidence of the RVFV-free status of this country.
Source: Tropical Animal Health and Production - Category: Veterinary Research Source Type: research
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