Diagnosis and prognosis potential of four gene promoter hypermethylation in prostate cancer.

This study assessed the differential methylation and mRNA expression between normal and prostate cancer samples. Correlation between promoter methylation and mRNA expression was estimated using Pearson's correlation coefficients. Moreover, the diagnostic potential of candidate methylation markers was estimated by the ROC curve using continuous beta values. Survival and Cox analysis was performed to evaluate the prognostic potential of the candidate methylation markers. A total of 359 hypermethylated sites 3435 hypomethylation sites, 483 upregulated genes, and 1341 downregulated genes were identified from the TCGA database. Furthermore, 17 hypermethylated sites (covering 13 genes), including known genes associated with hypermethylation in PCa (eg, AOX1 and C1orf114), showed high discrimination between adjacent normal tissues and PCa samples with AUCs from 0.88 to 0.94. Notably, ANXA2, FGFR2, HAAO, and KCNE3 were identified as valuable prognostic markers of PCa through the Kaplan-Meier analysis. Using gene methylation as a continuous variable, four promoter hypermethylation was significantly associated with DFS in univariate Cox regression and multivariate Cox regression. This study identified four novel diagnostic and prognostic markers for prostate cancer. The markers provide important strategies for improving the timely diagnosis and prognosis of PCa. This article is protected by copyright. All rights reserved. PMID: 32991011 [PubMed - as supplied by publisher]
Source: Cell Biology International - Category: Cytology Authors: Tags: Cell Biol Int Source Type: research