Sensors, Vol. 20, Pages 5594: Advances in Analysis of Milk Proteases Activity at Surfaces and in a Volume by Acoustic Methods

Sensors, Vol. 20, Pages 5594: Advances in Analysis of Milk Proteases Activity at Surfaces and in a Volume by Acoustic Methods
Sensors doi: 10.3390/s20195594
Authors:
Mark Dizon
Marek Tatarko
Tibor Hianik

This review is focused on the application of surface and volume-sensitive acoustic methods for the detection of milk proteases such as trypsin and plasmin. While trypsin is an important protein of human milk, plasmin is a protease that plays an important role in the quality of bovine, sheep and goat milks. The increased activity of plasmin can cause an extensive cleavage of β-casein and, thus, affect the milk gelation and taste. The basic principles of surface-sensitive acoustic methods, as well as high-resolution ultrasonic spectroscopy (HR-US), are presented. The current state-of-the-art examples of the application of acoustic sensors for protease detection in real time are discussed. The application of the HR-US method for studying the kinetics of the enzyme reaction is demonstrated. The sensitivity of the acoustics biosensors and HR-US methods for protease detection are compared.

Source: Sensors - September 28, 2020 Category: Biotechnology Authors: Mark Dizon Marek Tatarko Tibor Hianik Tags: Review Source Type: research