Phospholipase C gamma (PLC γ) regulates soluble trehalase in the 20E‐induced fecundity of Apolygus lucorum

In this study, 20E increasedAlPLC γ transcription as well as the abundance and activity of the encoded protein during molting and metamorphosis. The 20E treatment also induced the considerable accumulation of two second messengers, inositol triphosphate and diacylglycerol. The expression levels of genes encoding vitellogenin (AlVg) and soluble trehalase (AlTre ‐1) were similar to those ofAlPLC γ, and were upregulated in response to 20E. The silencing ofAlPLC γ resulted in downregulated expression ofAlTre ‐1 andAlVg. However, the silencing ofAlTre ‐1 andAlVg did not affectAlPLC γ expression. Moreover, the silencing ofAlVg did not alterAlTre ‐1 expression. Furthermore, an examination of the insect specimens indicated thatAlPLC γ is required for female adult reproduction, and that downregulated expression of this gene is associated with decreases in fecundity, adult longevity, and egg hatching rate as well as delayed oocyte maturation. We propose that 20E regulatesAlTre ‐1 expression viaAlPLC γ and affectsVg expression as well as ovary development to facilitate the reproductive activities ofA. lucorum females.
Source: Insect Science - Category: Biology Authors: Tags: ORIGINAL ARTICLE Source Type: research