Identification of salivary proteins in the whitefly Bemisia tabaci by transcriptomic and LC –MS/MS analyses

Graphical AbstractThe gene/protein repertoires ofBemisia tabaci salivary glands and secreted saliva were characterized by transcriptomic and LC –MS/MS analyses. A total of 698 salivary gland‐enriched unigenes and 171 salivary proteins were identified. AbstractThe whiteflyBemisia tabaci is a notorious agricultural pest of many crops worldwide. Although it is thought thatB. tabaci secretes saliva into the host plant to counter plant defenses, knowledge on the whitefly salivary proteome is limited. Here, we characterized the gene/protein repertoires ofB. tabaci salivary glands and secreted saliva by transcriptomic and liquid chromatography tandem mass spectroscopy analyses. A total of 698 salivary gland ‐enriched unigenes and 171 salivary proteins were identified. Comparative analysis between theB. tabaci salivary proteins and those of different arthropod species revealed numerous similarities in proteins associated with binding, hydrolysis, and oxidation ‐reduction, which demonstrates a degree of conservation across herbivorous saliva. There were 74 proteins only identified inB. tabaci saliva, of which 34 wereB. tabaci‐specific. In addition, 13 salivary proteins, of which 11 wereB. tabaci‐specific, were differentially regulated whenB. tabaci fed on different hosts. Our results provide a good resource for future functional studies of whitefly salivary effectors, and might be useful in pest management.

Source: Insect Science - August 25, 2020 Category: Biology Authors: Hai ‐Jian Huang, Zhuang‐Xin Ye, Gang Lu, Chuan‐Xi Zhang, Jian‐Ping Chen, Jun‐Min Li Tags: ORIGINAL ARTICLE Source Type: research