Sensors, Vol. 20, Pages 5572: A Surface Plasmon Resonance Biosensor Based on Directly Immobilized Hemoglobin and Myoglobin

Sensors, Vol. 20, Pages 5572: A Surface Plasmon Resonance Biosensor Based on Directly Immobilized Hemoglobin and Myoglobin Sensors doi: 10.3390/s20195572 Authors: Georgi Dyankov Ekaterina Borisova Evdokia Belina Hristo Kisov Ivan Angelov Alexander Gisbrecht Velichka Strijkova Nikola Malinowski Immobilization of proteins on a surface plasmon resonance (SPR) transducer is a delicate procedure since loss of protein bioactivity can occur upon contact with the untreated metal surface. Solution to the problem is the use of an immobilization matrix having a complex structure. However, this is at the expense of biosensor selectivity and sensitivity. It has been shown that the matrix-assisted pulsed laser evaporation (MAPLE) method has been successfully applied for direct immobilization (without a built-in matrix) of proteins, preserving their bioactivity. So far, MAPLE deposition has not been performed on a gold surface as required for SPR biosensors. In this paper we study the impact of direct immobilization of heme proteins (hemoglobin (Hb) and myoglobin (Mb)) on their bioactivity. For the purpose, Hb and Mb were directly immobilized by MAPLE technique on a SPR transducer. The bioactivity of the ligands immobilized in the above-mentioned way was assessed by SPR registration of the molecular reactions of various Hb/Mb functional groups. By SPR we studied the reaction between the beta chain of the Hb molecule and glucose, which shows the structural integrity of ...
Source: Sensors - Category: Biotechnology Authors: Tags: Article Source Type: research
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