Loop-mediated Isothermal Amplification (LAMP) for Identification of Pythium insidiosum

Loop-mediated isothermal amplification (LAMP), first introduced in 2000 by Notomi et al., is a simple, cost-effective, and efficient DNA amplification method (Notomi et al. 2000, 2015; Fu et al. 2011; Wong et al. 2018). Unlike most PCR-based techniques, LAMP runs under a single temperature condition, feasibly set by a heat block or water bath. LAMP enhances assay specificity and minimizes turnaround time by using a set of 4 or 6 gene-specific primers. Besides, it does not require sophisticated equipment (i.e., a thermocycler machine), making it an appealing method for many applications in resource-limited laboratories (Notomi et al.

Source: International Journal of Infectious Diseases - September 24, 2020 Category: Infectious Diseases Authors: Zin Mar Htun, Tiwa Rotchanapreeda, Thidarat Rujirawat, Tassanee Lohnoo, Wanta Yingyong, Yothin Kumsang, Pattarana Sae-Chew, Penpan Payattikul, Chompoonek Yurayart, Orawan Limsivilai, Piengchan Sonthayanon, Suthee Mangmee, Piriyaporn Chongtrakool, Theerapo Source Type: research