Lentinan improved the efficacy of vaccine against < i > Trichinella spiralis < /i > in an NLRP3 dependent manner
by Xuemin Jin, Xiaolei Liu, Jing Ding, Lixiao Zhang, Yaming Yang, Xuelin Wang, Yong Yang, Mingyuan Liu
There is an urgent need for the development of new, improved vaccine adjuvants againstT.spiralis infection. Polysaccharides are effective, safe, and biodegradable as adjuvant. In our study, we first observed the protective efficacy of lentinan as adjuvant against helminthT.spiralis infection. RecombinantT.spiralis Serpin (rTs-Serpin) immunoscreened from a cDNA library ofT.spiralis, as a vaccine, protect host againstTrichinella infection. The reduction rate of helminth burden of rTs-Serpin+lentinan –immunized mice was significantly increased compared with rTs-Serpin+FCA -immunized mice. rTs-Serpin+lentinan induced IgG1-dominant immune response and higher levels of IFN- γ and IL-4. rTs-Serpin+lentinan displayed a lower reduction rate of parasite burden in NLRP3-/- mice than that in WT mice and lower level of IgG1 than that in WT mice. The level of IL-4, but not IFN- γ, from NLRP3-/- mice immunized by rTs-Serpin+lentinan was significantly lower than that from WT mice, suggesting that NLRP3 is associated with rTs-Serpin+lentinan -triggering Th2 protective immunity againstT.spiralis infection. In summary, we revealed that lentinan was a novel adjuvant againstT.spiralis infection via NLRP3. NLRP3 therefore represents an important target for adjuvant discovery and the control ofT.spiralis infection.
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Xuemin Jin Source Type: research
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