Cancers, Vol. 12, Pages 2738: Schlafen 12 Is Prognostically Favorable and Reduces C-Myc and Proliferation in Lung Adenocarcinoma but Not in Lung Squamous Cell Carcinoma
Cancers, Vol. 12, Pages 2738: Schlafen 12 Is Prognostically Favorable and Reduces C-Myc and Proliferation in Lung Adenocarcinoma but Not in Lung Squamous Cell Carcinoma
Cancers doi: 10.3390/cancers12102738
Authors:
Sarmad Al-Marsoummi
Jonathan Pacella
Kaylee Dockter
Matthew Soderberg
Sandeep K. Singhal
Emilie E. Vomhof-DeKrey
Marc D. Basson
Schlafen 12 (SLFN12) is an intermediate human Schlafen that induces differentiation in enterocytes, prostate, and breast cancer. We hypothesized that SLFN12 influences lung cancer biology. We investigated survival differences in high versus low SLFN12-expressing tumors in two databases. We then adenovirally overexpressed SLFN12 (AdSLFN12) in HCC827, H23, and H1975 cells to model lung adenocarcinoma (LUAD), and in H2170 and HTB-182 cells representing lung squamous cell carcinoma (LUSC). We analyzed proliferation using a colorimetric assay, mRNA expression by RT-qPCR, and protein by Western blot. To further explore the functional relevance of SLFN12, we correlated SLFN12 with seventeen functional oncogenic gene signatures in human tumors. Low tumoral SLFN12 expression predicted worse survival in LUAD patients, but not in LUSC. AdSLFN12 modulated expression of SCGB1A1, SFTPC, HOPX, CK-5, CDH1, and P63 in a complex fashion in these cells. AdSLFN12 reduced proliferation in all LUAD cell lines, but not in LUSC cells. SLFN12 expression inversely correlated with expression of a myc-associated gene signature in LUAD, but not LUS...
Source: Cancers - Category: Cancer & Oncology Authors: Sarmad Al-Marsoummi Jonathan Pacella Kaylee Dockter Matthew Soderberg Sandeep K. Singhal Emilie E. Vomhof-DeKrey Marc D. Basson Tags: Article Source Type: research
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