Cancers, Vol. 12, Pages 2740: Modulation of Rat Cancer-Induced Bone Pain is Independent of Spinal Microglia Activity

Cancers, Vol. 12, Pages 2740: Modulation of Rat Cancer-Induced Bone Pain is Independent of Spinal Microglia Activity Cancers doi: 10.3390/cancers12102740 Authors: Marta Diaz-delCastillo Rie Bager Hansen Camilla Kristine Appel Lykke Nielsen Sascha Nolsøe Nielsen Konstantinos Karyniotakis Louise M. Dahl Rikke B. Andreasen Anne-Marie Heegaard The dissemination of cancer to bone can cause significant cancer-induced bone pain (CIBP), severely impairing the patient’s quality of life. Several rodent models have been developed to explore the nociceptive mechanisms of CIBP, including intratibial inoculation of breast carcinoma cells in syngeneic Sprague Dawley rats. Using this model, we investigated whether resident spinal microglial cells are involved in the transmission and modulation of CIBP, a long-debated disease feature. Immunohistochemical staining of ionizing calcium-binding adaptor molecule 1 (Iba-1) and phosphorylated p38-mitogen-activated protein kinase (P-p38 MAPK) showed no spinal microglial reaction in cancer-bearing rats, independently of disease stage, sex, or carcinoma cell line. As a positive control, significant upregulation of both Iba-1 and P-p38 was observed in a rat model of neuropathic pain. Additionally, intrathecal administration of the microglial inhibitor minocycline did not ameliorate pain-like behaviors in cancer-bearing rats, in contrast to spinal morphine administration. Our results indicate that microglial reactio...
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research