Immunohistochemistry or Molecular Analysis: Which Method Is Better for Subtyping Craniopharyngioma?

In this study, we investigated whether immunohistochemistry could replace genetic analysis as an aid in subtype diagnosis. Here, 38 CP patients who had undergone their first tumor resection were included. Among the 38 cases, 22 were morphologically diagnosed as ACP, 10 cases were diagnosed as PCP, and six cases were diagnosed as undetermined CP that were morphologically difficult to classify as either ACP or PCP. Results of immunohistochemistry and genetic analysis and clinical features were compared. Based on the immunohistochemistry, 26 (22 ACPs and four undetermined CPs) showed nuclear β-catenin expression, 11 (nine PCPs and two undetermined CPs) exhibited positive BRAF V600E immunostaining, and one PCP showed membranous β-catenin expression and negative BRAF V600E immunostaining. Among the 26 nuclear β-catenin expression cases, 11 hadCTNNB1 mutations; however, 15 cases had mutations of neitherCTNNB1 norBRAF V600E. All 11 BRAF V600E immunopositive cases hadBRAF V600E mutations. When comparing clinical features, pediatric patients and those with tumor calcification and less solid components on MRI more commonly had nuclear β-catenin expression tumors than BRAF V600E immunopositive tumors, reflecting the differences in clinical features between ACP and PCP. Accordingly, immunohistochemistry can replace genetic analysis as an aid to determine the subtype diagnosis of CP in general medical practice.
Source: Endocrine Pathology - Category: Pathology Source Type: research

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Conclusion. CP diagnosed in the elderly shows a similar distribut ion by sex and histologic forms than that diagnosed at younger ages. At presentation visual field alterations and headaches are the main clinical symptoms which improve substantially with surgery. However, surgery, mainly NTR, is accompanied by worsening of pituitary function, especially DI, which s eems to be a predictor of mortality in this population.
Source: Neuroendocrinology - Category: Endocrinology Source Type: research
This case report describes a case of hemorrhagic papilledema in a man diagnosed with a craniopharyngioma.
Source: JAMA Ophthalmology - Category: Opthalmology Source Type: research
Source: Acta Neuropathologica - Category: Neurology Source Type: research
CONCLUSIONS: Growth hormone treatment is a cause of pseudotumor cerebri syndrome. In our series, at risk patients had growth hormone deficiency and hypothalamic-pituitary anatomic anomalies or genetic or chromosome diseases. Fundus examination should be systematically screened in all patients in this at-risk group irrespective of the presence or not of symptoms. PMID: 33006547 [PubMed - as supplied by publisher]
Source: JCRPE Journal of Clinical Research in Pediatric Endocrinology - Category: Endocrinology Tags: J Clin Res Pediatr Endocrinol Source Type: research
Authors: Sprau A, Mahavadi A, Zhang M, Saste M, Deftos M, Singh H Abstract Background: Rathke's cleft cysts (RCCs) are benign, typically asymptomatic sellar lesions found incidentally in adults, with a dramatically lower incidence in pediatric patients (
Source: Surgical Neurology International - Category: Neurosurgery Tags: Surg Neurol Int Source Type: research
To determine the preirradiation baseline association of white matter integrity with neurocognitive function and to assess posttreatment changes in pediatric patients with craniopharyngioma treated with proton therapy.
Source: International Journal of Radiation Oncology * Biology * Physics - Category: Radiology Authors: Tags: Clinical Investigation Source Type: research
To determine the pre-irradiation baseline association of white matter integrity with neurocognitive function and to assess post-treatment changes in pediatric patients with craniopharyngioma treated with proton therapy.
Source: International Journal of Radiation Oncology * Biology * Physics - Category: Radiology Authors: Source Type: research
AbstractSince there are many approaches for successful craniopharyngioma resection, how to choose a suitable approach remains problematic. The aim of this study was to summarize experience of approach selection and outcomes of craniopharyngioma resection in our institute. The data of 182 primary craniopharyngiomas between January 2013 and June 2019 were retrospectively reviewed. Craniopharyngiomas were classified into intrasellar, intra-suprasellar, suprasellar, and intra-third ventricle types based on the location. The surgical approaches, extent of resection, endocrine and ophthalmological outcomes, and complications wer...
Source: Neurosurgical Review - Category: Neurosurgery Source Type: research
Publication date: December 2020Source: Interdisciplinary Neurosurgery, Volume 22Author(s): Baha'eddin A. Muhsen, Bilal Ibrahim, Edinson Najera, Hamid Borghei-Razavi, Badih Adada
Source: Interdisciplinary Neurosurgery - Category: Neurosurgery Source Type: research
We examined a 110-week-old RccHanTM: WIST Wistar male rat from a carcinogenicity study. No clinical signs were observed, and the rat was sacrificed at the end of the study. Macroscopically, within the midline of the sphenoid bone, was a 10 mm, non-infiltrative, soft, heterogeneous mass. Microscopic evaluation showed an expansile, cystic proliferation, consisting of two patterns of epithelial lining: well-differentiated areas lined by a single layer to a pseudostratified, ciliated-cuboidal epithelia with Goblet cells compatible with Rathke's cleft cyst; and poorly differentiated ones that formed irregular papillary projecti...
Source: Journal of Toxicologic Pathology - Category: Toxicology Tags: J Toxicol Pathol Source Type: research
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