Mouse models for assessing the cross-protective efficacy of oral non-typhoidal Salmonella vaccine candidates harboring in-frame deletions of the ATP-dependent protease lon and other genes.

Mouse models for assessing the cross-protective efficacy of oral non-typhoidal Salmonella vaccine candidates harboring in-frame deletions of the ATP-dependent protease lon and other genes. J Med Microbiol. 2015 Jan 14; Authors: Matsui H, Fukiya S, Kodama-Akaboshi C, Eguchi M, Yamamoto T Abstract In BALB/c mouse models of Salmonella enterica serovar Typhimurium (S. Typhimurium) infection, a single oral immunization with a mutant strain with an insertion of the chloramphenicol resistance gene into the ATP-dependent protease clpP or lon gene decreased the number of salmonellae in each tissue sample five days after oral challenge with virulent S. Typhimurium at weeks 26 and 54 postimmunization. These data suggest that an oral immunization with the ClpP- or Lon-disrupted S. Typhimurium strain can provide long-term protection against oral challenge with virulent S. Typhimurium. Accordingly, recombinant oral non-typhoidal Salmonella (NTS) vaccines were constructed by incorporating mutants of both S. Typhimurium and S. enterica serovar Enteritidis (S. Enteritidis) harboring stable in-frame markerless deletions of the clpP-lon-sulA (suppressor of lon), lon-sulA, or lon-msbB (acyltransferase) genes. Among these orally administered vaccine candidates, those with the lon-sulA gene deletion mutants of S. Typhimurium and S. Enteritidis protected BALB/c and C57BL/6J mice against oral challenge with both virulent S. Typhimurium and virulent S. Enter...
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research