Comparison of loop-mediated isothermal amplification and real-time PCR for detecting Bordetella pertussis.

Comparison of loop-mediated isothermal amplification and real-time PCR for detecting Bordetella pertussis.
J Med Microbiol. 2015 Jan 16;
Authors: Allahyar Torkaman MR, Kamachi K, Nikbin VS, Lotfi MN, Shahcheraghi F
Abstract
Loop-mediated isothermal amplification (LAMP) is a rapid, sensitive and specific technique for detecting various infectious diseases. In the present study, we developed a LAMP assay based on insertion sequence IS481 of Bordetella pertussis (IS481-LAMP). We performed a LAMP assay of the PT promoter region of B. pertussis (PT-LAMP), and the results were compared with those obtained using a conventional real-time PCR based on IS481. Compared with the real-time PCR assay, the specificity of PT-LAMP and IS481-LAMP were 94.1% and 92.9%, and sensitivity were 76.2% and 87.5%, respectively. Therefore, real-time PCR is the most reliable method for detecting B. pertussis. However, PT-LAMP and IS481-LAMP may be alternative methods that can be used for the diagnosis of B. pertussis in clinical laboratories.
PMID: 25596118 [PubMed - as supplied by publisher]

Source: Journal of Medical Microbiology - January 16, 2015 Category: Microbiology Authors: Allahyar Torkaman MR, Kamachi K, Nikbin VS, Lotfi MN, Shahcheraghi F Tags: J Med Microbiol Source Type: research