Evaluation of the Speed-Oligo® Mycobacteria assay for the identification of nontuberculous mycobacteria.

We examined a total of 62 strains (9 type strains, 19 reference strains and 34 clinical isolates). Speed-Oligo® Mycobacteria assay was performed according to the manufacturer's instructions. Discrepant results between Speed-Oligo® Mycobacteria and the original identification were re-assessed by the Speed-Oligo® Mycobacteria and resolved by the GenoType Mycobacterium CM assay and by sequencing of 16S rRNA and protein encoding genes. We found 93.5% (58/62) of concordance for the identification of NTM as compared to the original identification. Three strains were erroneously identified by Speed-Oligo® Mycobacteria: one Mycobacterium kansasii was identified as M. tuberculosis complex and one Mycobacterium chelonae and Mycobacterium peregrinum both identified as Mycobacterium abscessus. Moreover, one Mycobacterium chelonae strain was not identified by Speed-Oligo® Mycobacteria since it did not react with any species-specific probe. For these strains, sequencing of the genes hsp65, 16S rRNA, and rpoB and the GenoType Mycobacterium CM assay were performed. The Speed-Oligo® Mycobacteria assay can be a useful tool for the rapid and easy identification of the most common NTM. If applied in the clinical practice it could reduce diagnostic delays and contribute to correct clinical and better management of infections caused by NTM. PMID: 25596120 [PubMed - as supplied by publisher]
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research