Cancers, Vol. 12, Pages 2709: Bortezomib-Loaded Mesoporous Silica Nanoparticles Selectively Alter Metabolism and Induce Death in Multiple Myeloma Cells

Cancers, Vol. 12, Pages 2709: Bortezomib-Loaded Mesoporous Silica Nanoparticles Selectively Alter Metabolism and Induce Death in Multiple Myeloma Cells Cancers doi: 10.3390/cancers12092709 Authors: Alessandra Nigro Luca Frattaruolo Mariarosa Fava Ilaria De Napoli Marianna Greco Alessandra Comandè Marzia De Santo Michele Pellegrino Elena Ricci Francesca Giordano Ida Perrotta Antonella Leggio Luigi Pasqua Diego Sisci Anna Rita Cappello Catia Morelli A mesoporous silica-based nanodevice bearing the antineoplastic drug bortezomib (BTZ), whose release is triggered in acidic environment and grafted with folic acid (FOL) as a targeting function (FOL-MSN-BTZ) was tested on folate receptor overexpressing (FR+) multiple myeloma (MM) cells and on FR negative (FR−) normal cells. FOL-MSN-BTZ efficacy studies were conducted by means of growth experiments, TEM, TUNEL assay and Western Blotting analysis (WB). Metabolic investigations were performed to assess cells metabolic response to MSNs treatments. FOL-MSN-BTZ exclusively killed FR+ MM cells, leading to an apoptotic rate that was comparable to that induced by free BTZ, and the effect was accompanied by metabolic dysfunction and oxidative stress. Importantly, FOL-MSN-BTZ treated FR− normal cells did not show any significant sign of injury or metabolic perturbation, while free BTZ was still highly toxic. Notably, the vehicle alone (MSN-FOL) did not affect any biological pro...
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research