A method for the quantitative detection of Cas12a ribonucleoproteins

Chem. Commun., 2020, Advance Article DOI: 10.1039/D0CC04019C, CommunicationJie Qiao, Siyu Lin, Wenli Sun, Lixin Ma, Yi Liu We developed a method for the rapid and quantitative detection of the effective Cas12a RNPs by the combined use of anti-CRISPR protein AcrVA1 and stem-loop RT-qPCR, achieving a limit of detection (LOD) of 1 fM. To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry

Source: RSC - Chem. Commun. latest articles - September 21, 2020 Category: Chemistry Authors: Jie Qiao Source Type: research