Long non ‑coding RNA SNHG16 inhibits the oxygen‑glucose deprivation and reoxygenation‑induced apoptosis in human brain microvascular endothelial cells by regulating miR‑15a‑5p/bcl‑2.

Long non‑coding RNA SNHG16 inhibits the oxygen‑glucose deprivation and reoxygenation‑induced apoptosis in human brain microvascular endothelial cells by regulating miR‑15a‑5p/bcl‑2. Mol Med Rep. 2020 Jul 29;: Authors: Teng H, Li M, Qian L, Yang H, Pang M Abstract MicroRNA (miR) 15a‑5p can promote ischemia/reperfusion (I/R)‑induced apoptosis of cerebral vascular endothelial cells, which is inhibited by long non‑coding RNAs (lncRNAs). The present study investigated the potential of lncRNAs targeting miR‑15a‑5p to regulate oxygen‑glucose deprivation and reoxygenation (OGD‑R)‑induced apoptosis of human brain microvascular endothelial cells (hBMECs). hBMECs were transfected with or without miR‑15a‑5p or its mutant, together with p‑small nucleolar RNA host gene 16 (SNHG16) or its mutant. Following OGD‑R, proliferation, apoptosis and miR‑15a‑5p, SNHG16 and Bcl‑2 expression levels were determined using MTT, flow cytometry, reverse transcription‑quantitative PCR or western blotting. The potential interaction of SNHG16 with miR‑15a‑5p was analyzed by pull‑down, luciferase and immunoprecipitation assays. OGD‑R induced apoptosis of hBMECs and increased miR‑15a‑5p expression levels in a time‑dependent manner. miR‑15a‑5p overexpression decreased the proliferation of hBMECs and promoted apoptosis by decreasing Bcl‑2 expression levels. SNHG16 was pulled‑down by miR‑15a‑5p and anti...
Source: Molecular Medicine Reports - Category: Molecular Biology Tags: Mol Med Rep Source Type: research