Complement activation in human autoimmune diseases and mouse models; employing a sandwich immunoassay specific for C3dg.

We present a stable, reproducible, and easy-to-use, time-resolved immunoassay with specificity for C3dg that can be used to directly evaluate ongoing complement activation. We demonstrate that the assay can be applied to clinical samples with a high specificity (95%) and a positive likelihood ratio of 10. It can also differentiate the complement related disease Systemic Lupus Erythematosus from controls and other immune-mediatedimmune mediated diseases like Rheumatoid Arthritis (86% specificity) and Spondyloarthritis (91% specificity).. Further, we establish how the assay may also be used for experimental research in in vivo mouse models.
PMID: 32941885 [PubMed - as supplied by publisher]

Source: Journal of Immunological Methods - September 13, 2020 Category: Allergy & Immunology Authors: Troldborg A, Halkjær L, Pedersen H, Hansen A, Loft AG, Lindegaard H, Stengaard-Pedersen K, Graversen JH, Palarasah Y, Thiel S Tags: J Immunol Methods Source Type: research