Bacterial Whole Genome Sequencing on the Illumina iSeq 100 for Clinical and Public Health Laboratories

Bacterial whole-genome sequencing (WGS) provides clinical and public health laboratories an unprecedented level of information on species identification, antimicrobial resistance, and epidemiological typing. However, multiple barriers to widespread adoption still exist. Here, we describe bacterial WGS using the Illumina iSeq 100 instrument to overcome some of these barriers. Using an in-house, high quality single nucleotide polymorphism (hqSNP) analysis pipeline and the BIOM ÉRIEUX EPISEQ CS whole-genome Multilocus Sequence Typing (wgMLST) program, we validated the sequencing of Acinetobacter baumannii, Burkholderia cepacia, Clostridoides difficile, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, Serratia marcescens, and Staphylococcus aureus isolates.
Source: Journal of Molecular Diagnostics - Category: Pathology Authors: Tags: Regular Article Source Type: research