Cancers, Vol. 12, Pages 2676: Evaluation of the Role of ITGBL1 in Ovarian Cancer

Cancers, Vol. 12, Pages 2676: Evaluation of the Role of ITGBL1 in Ovarian Cancer Cancers doi: 10.3390/cancers12092676 Authors: Alexander Jorge Cortez Katarzyna Aleksandra Kujawa Agata Małgorzata Wilk Damian Robert Sojka Joanna Patrycja Syrkis Magdalena Olbryt Katarzyna Marta Lisowska In our previous microarray study we identified two subgroups of high-grade serous ovarian cancers with distinct gene expression and survival. Among differentially expressed genes was an Integrin beta-like 1 (ITGBL1), coding for a poorly characterized protein comprised of ten EGF-like repeats. Here, we have analyzed the influence of ITGBL1 on the phenotype of ovarian cancer (OC) cells. We analyzed expression of four putative ITGBL1 mRNA isoforms in five OC cell lines. OAW42 and SKOV3, having the lowest level of any ITGBL1 mRNA, were chosen to produce ITGBL1-overexpressing variants. In these cells, abundant ITGBL1 mRNA expression could be detected by RT-PCR. Immunodetection was successful only in the culture media, suggesting that ITGBL1 is efficiently secreted. We found that ITGBL1 overexpression affected cellular adhesion, migration and invasiveness, while it had no effect on proliferation rate and the cell cycle. ITGBL1-overexpressing cells were significantly more resistant to cisplatin and paclitaxel, major drugs used in OC treatment. Global gene expression analysis revealed that signaling pathways affected by ITGBL1 overexpression were mostly those related to extracellular...
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research