Amyloid precursor protein 770 is specifically expressed and released from platelets [Glycobiology and Extracellular Matrices]

In this study, we used peripheral blood samples from patients with CAD and control subjects and evaluated sAPP770 as a specific biomarker for platelet activation. First, the plasma levels of sAPP770 correlated well with those of the soluble form CD40 ligand (CD40L), an established biomarker for platelet activation. Additionally, flow cytometry analysis using peripheral blood cells showed that CD40L expression is up-regulated in activated T cells, whereas APP770 expression is negligible in all blood cell types except platelets. Following stimulation with collagen or ADP, aggregating platelets immediately released sAPP770. Finally, patients with dual antiplatelet therapy showed significantly lower levels of plasma sAPP770 than those with no therapy. Taken together, our data show that plasma sAPP770 could be a promising biomarker for platelet activation.

Source: Journal of Biological Chemistry - September 17, 2020 Category: Chemistry Authors: Saori Miura, Akiomi Yoshihisa, Tomofumi Misaka, Takayoshi Yamaki, Takao Kojima, Masahiro Toyokawa, Kazuei Ogawa, Hiroki Shimura, Naomasa Yamamoto, Kohji Kasahara, Yasuchika Takeishi, Shinobu Kitazume Tags: Molecular Bases of Disease Source Type: research