Long-Term Mosquito culture with SkitoSnack, an artificial blood meal replacement

by Yashoda Kandel, Soumi Mitra, Xavier Jimenez, Stacy D. Rodriguez, Alvaro Romero, Brittny N. Blakely, Sang Yeon Cho, Charles Pelzman, Immo A. Hansen The reliance on blood is a limiting factor for mass rearing of mosquitoes for Sterile-Insect-Technique (SIT) and other mosquito-based control strategies. To solve this problem, we have developed SkitoSnack, a formulated diet forAedes aegypti (L) mosquitoes, as an alternative for vertebrate blood. Here we addressed the question if long-term yellow fever mosquito culture with SkitoSnack resulted in changed life history traits and fitness of the offspring compared to blood-raised mosquitoes. We also explored if SkitoSnack is suitable to raise Asian tiger mosquitos,Aedes albopictus (L.), and the human bed bug,Cimex lectularius (L). We measured life history traits for 30th generation SkitoSnack-raisedAe.aegypti and 11th generation SkitoSnack-raisedAe.albopictus, and compared them with control mosquitoes raised on blood only. We compared meal preference, flight performance, and reproductive fitness inAe.aegypti raised on SkitoSnack or blood. We also offered SkitoSnack to bed bug nymphs. We found that long-term culture with SkitoSnack resulted in mosquitoes with similar life history traits compared to bovine blood-raised mosquitoes in both species we studied. Also,Ae.aegypti mosquitoes raised on SkitoSnack had similar flight performance compared to blood raised mosquitoes, were still strongly attracted by human smell and had equal mat...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research