Rational design of in situ localization solid-state fluorescence probe for bio-imaging of intracellular endogenous cysteine.

Rational design of in situ localization solid-state fluorescence probe for bio-imaging of intracellular endogenous cysteine.
Talanta. 2020 Dec 01;220:121364
Authors: Liu Y, Wu YX, Zhang D, Zhong H, Li D, He K, Wei WT, Yu S
Abstract
Fluorescence detection technology has been widely concerned for its advantages of low cost, simple operation, good sensitivity, real-time and non-destructive biological imaging. However, most fluorophores emit bright fluorescence in solution, and the fluorescence decreases significantly in the high concentration or solid/aggregated state, which is called aggregation-caused quenching (ACQ). Cysteine (Cys) is an important kind of amino-acid in the field of bio-medicine, whose main function is to participate in metabolism and protein synthesis, detoxification, but intracellular cysteine concentrations (30-200 μM) are much low, and direct detection of endogenous cysteine is hampered by interference with other thiols. To solve the above problems, based on solid-state fluorophore HPQ, we for the first time prepared a novel solid-state fluorescence probe MA-HPQ, for monitoring of endogenous Cys, operated by the mechanism of excited intramolecular proton transfer (ESIPT). MeO-HPQ is completely insoluble in water, has very strong solid-state fluorescence with the maximum emission wavelength of 510 nm and the maximum excitation wavelength of 365 nm. This special property makes it very suitable for confocal micro...

Source: Talanta - September 17, 2020 Category: Chemistry Authors: Liu Y, Wu YX, Zhang D, Zhong H, Li D, He K, Wei WT, Yu S Tags: Talanta Source Type: research