An europium functionalized carbon dot-based fluorescence test paper for visual and quantitative point-of-care testing of anthrax biomarker.
We reported Eu(III) functionalized carbon dots (CDs-Eu) as a novel ratiometric fluorescent probe for point-of-care testing (POCT) of dipicolinic acid (DPA), a biomarker of anthrax. The probe was synthesized simply by coordination interaction between Eu(III) ions and the CDs with carboxyl and amino groups. Upon addition of DPA, the intrinsic red luminescence of Eu(III) ions can be sensitized due to the presence of energy transfer when DPA chromophore coordinated with Eu(III) ions. The fluorescence peaking at 530 nm as a reference remained essentially constant, leading to ratiometric ﬂuorescence response toward DPA. This approach exhibited a linearity range from 0.5 nM to 5 μM and a detection limit of 0.8 nM. More Importantly, the combination of probe-based test paper and smartphone provided a convenient approach for visual POCT of DPA with a detection limit of 67 nM, which was lower than other reported test paper for DPA detection. The color of fluorescence gradually changed from green to red as the DPA increases, which can be captured by a smartphone and further quantitatively analyzed with a built-in Color Analyzer App. The satisfactory result of detecting DPA accurately and reliably in real samples by the test paper certified its practicality in POCT of DPA. PMID: 32928403 [PubMed - in process]
Conclusion: This study shows that there is a knowledge gap about anthrax among the people in the affected communities. Key drivers for the anthrax outbreak such as poor cultural beliefs and practices and wildlife-livestock-human interactions were observed in all the three subcounties studied. All these findings could imply a high risk of outbreak of anthrax in Arua and Ugandan agricultural communities where the public health programs are less standardized and less effective. PMID: 33014075 [PubMed]
This study aimed at detecting and profiling antibiotic resistance of bacterial contaminants in vended food and the environment. Seventy antibiotic-resistant bacterial isolates were isolated from fried fish, African sausages, roasted meat, smokies, samosa, chips (potato fries), vegetable salads, and soil samples collected from Embu Town and Kangaru Market in Embu County, Kenya. The antibiotic susceptibility test, morphological and biochemical characterization, antibiosis assay, polymerase chain reaction-based detection of antibiotic resistance genes, and sequencing of the 16S rRNA gene were done. Analysis of variance on all...
Publication date: Available online 23 September 2020Source: Preventive Veterinary MedicineAuthor(s): Ayalew Assefa, Amare Bihon, Abebe Tibebu
In this study, a proba bilistic framework is developed that extends Brookmeyer's competing‐risks dose–response model to allow for variation in factors such as dose‐dispersion, dose‐deposition, and other within‐host parameters. With data sets drawn from dose–response experiments of inhalational anthrax, plague, and tularemia, we illustrate how for certain cases, there is the potential for overestimation of infection numbers arising from models that consider only the experimental data in isolation.
Background: Anthrax continues to be a high priority pathogen in Uganda because of its potential for severe economic harm to livestock and humans. It is distributed globally and remains enzootic in many regions of the world, particularly sub-Saharan Africa, Asia, and Central and South America (WHO, 2008).
Background: Anthrax is a zoonotic infection caused by the bacteria Bacillus anthracis. Humans acquire cutaneous infection through contact with infected animals or animal products. During May 2018, a sub-country chief in Kiruhura District received reports of humans with suspected cutaneous anthrax occurring subsequent to the sudden death of three cows on a farm on May 6. The human patients had reportedly participated in the butchery and consumption of meat from the dead cows; specimens from the animal carcasses tested positive for B.
(Natural News) The Wuhan coronavirus continues to be one for the books, much like the anthrax attacks of 2001 or the financial crisis of 2008. However, these earlier crises that rocked the country were isolated, with one a national security crisis while the other was a financial crisis. These events were more limited in scope...
by Olubunmi R. Aminu, Tiziana Lembo, Ruth N. Zadoks, Roman Biek, Suzanna Lewis, Ireen Kiwelu, Blandina T. Mmbaga, Deogratius Mshanga, Gabriel Shirima, Matt Denwood, Taya L. Forde Anthrax threatens human and animal health, and people’s livelihoods in many rural communities in Africa and Asia. In these areas, anthrax surveillance is challenged by a lack of tools for on-site detection. Furthermore, cultural practices and infrastructure may affect sample availability and qua lity. Practical yet accurate diagnostic solutions are greatly needed to quantify anthrax impacts. We validated microscopic and molecular methods fo...
Publication date: Available online 12 September 2020Source: Journal of Microbiological MethodsAuthor(s): Fatemeh Hoorali, Hossein Khosravi, Bagher Moradi
Analytical ChemistryDOI: 10.1021/acs.analchem.0c01570