In situ functionalization of HPLC monolithic columns based on divinylbenzene-styrene-4-vinylbenzyl chloride.

In this study, the surface treatment of monolithic sorbent based on divinylbenzene (DVB), styrene (St) and 4-vinylbenzyl chloride (4VBC) copolymer by heterocyclic nitrogen-containing compounds 1-methylimidazole (1MI), 2-methylimidazole (2MI), 2-methylpyridine (2 MP) and 4-methylpyridine (4 MP) is described. The reaction of nitrogen-containing heterocycles with chloromethyl fragments on the surface results in formation of ion pairs and significantly changes the selectivity of monolithic columns. The chromatographic properties of prepared columns are studied. Modified columns can be operated in reversed-phase (RP) chromatography or in hydrophilic interaction liquid chromatography (HILIC) with different composition of the mobile phase. Separation examples of various chemical substances classes are given.
PMID: 32928418 [PubMed - in process]

Source: Talanta - September 17, 2020 Category: Chemistry Authors: Sotnikova YS, Patrushev YV, Sidelnikov VN, Mazaeva AA Tags: Talanta Source Type: research