Immobilization of recombinant lysostaphin on nano ‐particle through biotin‐streptavidin conjugation technology as a geometrical progressed confrontation against staphylococcus aureus infection

This study aimed to design a hyper valency TiO2 nanocomposite as a reserved base to carry a high amount of active antibacterial agents such as lysostaphin via a biotin ‐streptavidin‐biotin bridge. The utilization of the Streptavidin‐Biotin system could increase the abundance of lysostaphin. Lysostaphin was expressed inE.coli and purified. Both recombinant lysostaphin and titanium oxide nanocomposite were conjugated with Biotin and linked to streptavidin bridge. The kinetic and activity of the enzyme were examined after each step utilizing N ‐acetylhexaglycine as a substrate. Physical characteristics of nanoparticles containing lysostaphin were determined using AFM, SEM, FTIR, and Zeta potential. The results showed changes in size, charge, and morphology of the nanoparticles following lysostaphin attachment. Also, the stability and ki netics of the active biological enzymes on nanoparticles were re‐examined following eight months of storage. Exploiting this approach, various biotinylated antibacterial agents could be prepared and rapidly immobilized on nanoparticle as an active net against related infectious agents.This article is protected by copyright. All rights reserved
Source: Biotechnology and Applied Biochemistry - Category: Biochemistry Authors: Tags: Original Article Source Type: research