Monoclonal antibody 4B1 influences the pKa of Glu325 in lactose permease (LacY) from Escherichia coli: evidence from SEIRAS
In this study, surface ‐enhanced infrared absorption spectroscopy applied to the immobilized LacY was used to study the pH‐dependent changes in LacY and to accessin situ the effect of the 4B1 antibody on the pKa of Glu325, the primary functional H+‐binding site in LacY. A small shift of the pK value from 10.5 to 9.5 was identified that can be corroborated with the inactivation of LacY upon 4B1 binding.
Source: FEBS Letters - Category: Biochemistry Authors: Fatima Omeis,
Ana Filipa Santos Seica,
Natalia Ermolova,
H. Ronald Kaback,
Petra Hellwig Tags: Research Letter Source Type: research