Has-miR-17 increases the malignancy of gastric lymphoma by HSP60/TNFR2 pathway.
Has-miR-17 increases the malignancy of gastric lymphoma by HSP60/TNFR2 pathway.
J Biol Regul Homeost Agents. 2020 Jul-Aug;34(4):1317-1324
Authors: Wang LL, Dong JJ, An BZ, Liang J, Cai KR, Jin ZS, Jin HS, Hu JP
Abstract
The purpose of this study was to investigate the expression and mechanism of miR-17 in gastric lym-phoma. miR-17mimics, miR-17 inhibitors and negative controls were transfected into human gastric lymphoma cell line cyp6d. The proliferation, invasion and apoptosis of cyp6d cells were detected by CCK-8, Transwell and TUNEL methods, respectively. The expression and clinicopathological features of miR-17 in gastric lymphoma were analyzed by real-time quantitative PCR. The target gene of miR-17 was predicted by targetscan 7.2, and the expression of miR-17 related protein was detected by Western blot. The results showed that the expression of miR-17 in gastric lymphoma was significantly higher than that in normal tissues (P < 0.05), which was closely related to lymph node metastasis, tumor size and distant metastasis (P < 0.05). The high expression of miR-17 significantly promoted the proliferation and invasion of cyp6d cells and inhibited apoptosis (P < 0.05). The high expression of miR-17 can regu¬late the expression of HSP60 and TNFR2. It has been found that miR-17 can promote the development of gastric lymphoma by regulating HSP60/TNFR2 pathway, which is a potential molecular target for the diagnosis and treat...
Source: Journal of Biological Regulators and Homeostatic Agents - Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research
More News: Biomedical Science | Cancer & Oncology | Gastroenterology | Genetics | Lymphoma | Molecular Biology | Study