Pseudomonas donghuensis HYS 7-hydroxytropolone contributes to pathogenicity toward Caenorhabditis elegans and is influenced by pantothenic acid.

Pseudomonas donghuensis HYS 7-hydroxytropolone contributes to pathogenicity toward Caenorhabditis elegans and is influenced by pantothenic acid. Biochem Biophys Res Commun. 2020 Sep 10;: Authors: Gui Z, You J, Xie G, Qin Y, Wu T, Xie Z Abstract Pseudomonas donghuensis HYS, a bacterial strain identified from Donghu Lake, has tremendous toxicity toward Caenorhabditis elegans and is characterized by high 7-hydroxytropolone siderophore production. Here, the relationship between pathogenic siderophore production and pantothenic acid was evaluated. The pathogenicity of P. donghuensis HYS was illustrated using C. elegans as a host. Based on slow-killing assay findings, a 7-hydroxytropolone deficiency-causing mutation attenuated P. donghuensis HYS pathogenicity, which was restored by the addition of extracted 7-hydroxytropolone. Moreover, data from real-time qPCR analysis and characteristic absorption assays indicated that pantothenic acid deficiency repressed transcriptional levels of orf9, which further reduced 7-hydroxytropolone production. Furthermore, slow-killing assays indicated that panB and pantothenic acid affected the virulence of P. donghuensis. These results indicate that a 7-hydroxytropolone siderophore-producing strain is virulent toward C. elegans. Our findings demonstrate that pantothenic acid is associated with P. donghuensis siderophore production-related pathogenicity. PMID: 32921415 [PubMed - as supplied by ...
Source: Biochemical and Biophysical Research communications - Category: Biochemistry Authors: Tags: Biochem Biophys Res Commun Source Type: research