Characterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil.

Characterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil. Infect Genet Evol. 2020 Sep 10;:104550 Authors: Rabahi MF, Conceição EC, de Paiva LO, Souto MVML, Sisco MC, de Waard J, de Souza Caldas PC, Fandinho F, Ramos JP, de Carvalho LD, Campos CED, Lima KVB, da Silva SP, Sharma A, Robledo J, Paez UAH, Duarte RS, da Silva MG, Gomes LL, Vasconcellos SEG, Uwezeye C, de Jong BC, Junqueira-Kipnis AP, Suffys PN Abstract Human tuberculosis (TB) is caused by members of the Mycobacterium tuberculosis complex (MTBC), including Mycobacterium tuberculosis var. tuberculosis (MTB) and Mycobacterium tuberculosis var. africanum (MAF). While MTB is isolated worldwide, MAF is almost completely restricted to the African continent, and despite the historical proximity between Brazil and Africa during the slave trade, no case of TB being caused by MAF has been reported in Brazil to date. We hereby describe the first case of TB caused by MAF in Brazil comparing its genome against the published ones. A female patient who had never visited Africa presented with clinical symptoms typical of pulmonary TB. Based on 16S rRNA gene sequencing, the cultured isolate was identified as belonging to MTBC and partial sequence of the hsp65 gene was identical to that of MAF. This was confirmed by genotyping based on detection of Single Nucleotide Polymorphism (SNP), Region of Difference (RD) and spoligot...
Source: Infection, Genetics and Evolution - Category: Genetics & Stem Cells Authors: Tags: Infect Genet Evol Source Type: research