Molecular detection of Anaplasma spp. in domestics dogs from urban areas of Soledad, Atlantico, Colombia.

Molecular detection of Anaplasma spp. in domestics dogs from urban areas of Soledad, Atlantico, Colombia. Infez Med. 2020 Sep 01;28(3):373-383 Authors: Bonilla-Aldana DK, Pomares-Cantillo LH, Beltrán-Sánchez CA, Bettin-Martínez AC, Campo-Urbina ML, Rodriguez-Morales AJ, Pérez-Doria A Abstract Tick-borne pathogens are etiological agents of some zoonotic diseases, causing important consequences in animal and human health. These are emerging around the world, especially in tropical countries including Colombia. Domestic dogs play an essential role in the epidemiology of several zoonotic tick-borne pathogens. We performed the detection of bacteria from Anaplasmataceae family and parasites from the Piroplasmida order, in 85 domestic dogs from Soledad municipality, Atlantico, Colombia. Peripheral blood smears, detection by duplex PCR assay (ss rRNA 16S, from bacteria and the ITS-1, of ribosomal DNA from parasites), and DNA sequencing by Sanger method were done. Taxonomic identification was made by phylogenetics analysis of the DNA sequences. The gene sequences analysis showed that 12.9% of the dogs were infected with Anaplasma spp. Infection was higher in young dogs (OR=4.72, 95%CI 1.267-17.584). Besides that, 3.5% of them showed inclusions (morulae) compatible with bacteria from the order Rickettsiales. A coinfection with Babesia spp. and a Rickettsiales bacterial pathogens was found. The frequency of Anaplasma spp. detected in domest...
Source: Infezioni in Medicina - Category: Infectious Diseases Tags: Infez Med Source Type: research