CircMMP1 promotes the progression of glioma through miR ‐433/HMGB3 axis in vitro and in vivo

AbstractCircular RNAs (circRNAs) are non ‐coding RNAs that have shown to regulate the progression of human diseases, including a variety of cancers. We aimed to investigate the function and the underlying working mechanism of circRNA matrix metallopeptidase 1 (circMMP1; hsa_circ_0024108) in glioma progression. Quantitative real‐time po lymerase chain reaction (qRT‐PCR) was performed to examine the expression of circMMP1, microRNA‐433‐3p (written as “miR‐433”) and high mobility group box 3 (HMGB3). Nanoparticle tracking analysis (NTA) was used to analyze the relative concentration and size distribution of serum exosomes . Cell proliferation was analyzed via cell counting kit‐8 (CCK8) assay and colony formation assay. Transwell migration and invasion assays were used to examine the metastasis ability of glioma cells. Cell apoptosis was assessed by flow cytometry. Dual‐luciferase reporter assay and RNA immunoprec ipitation (RIP) assay were used to verify the interactions of circMMP1/miR‐433 and miR‐433/HMGB3 messenger RNA (mRNA). Xenograft mouse model was built to clarify the role of circMMP1 in vivo. The results showed that high serum exosomal circMMP1 level might predict poor prognosis of glioma patien ts. CircMMP1 promoted the proliferation and motility and impeded the apoptosis of glioma cells. MiR‐433 was a direct target of circMMP1, and circMMP1 silencing‐mediated influences in glioma cells were partly alleviated by the knockdown of miR‐433. M...
Source: IUBMB Life - Category: Research Authors: Tags: RESEARCH COMMUNICATION Source Type: research