Effects of benzene on gilts ovarian cell functions alone and in combination with buckwheat, rooibos, and vitex.

Effects of benzene on gilts ovarian cell functions alone and in combination with buckwheat, rooibos, and vitex. Environ Sci Pollut Res Int. 2020 Sep 11;: Authors: Sirotkin AV, Macejková M, Tarko A, Fabova Z, Alrezaki A, Alwasel S, Harrath AH Abstract We aimed to examine the influence of benzene and of three dimethyl sulfoxide (DMSO) plant extracts-buckwheat (Fagopyrum Esculentum), rooibos (Aspalathus linearis), and vitex, (Vitex Agnus-Castus), and the combination of benzene with these three plant extracts on basic ovarian cell functions. Specifically, the study investigated the influence of benzene (0, 10, 100, or 1000 ng/mL) with and without these three plant additives on porcine ovarian granulosa cells cultured during 2 days with and without these additives. Cell viability, proliferation (accumulation of proliferating cell nuclear antigen, PCNA), apoptosis (accumulation of Bcl-2-associated X protein , bax), and the release of progesterone (P) and estradiol (E) were analyzed by the Trypan blue test, quantitative immunocytochemistry, and enzyme-linked immunosorbent assay, respectively. Benzene reduced cell viability, as well as P and E release. Plant extracts, given alone, were able directly promote or suppress ovarian cell functions. Furthermore, buckwheat and rooibos, but not vitex prevented the inhibitory action of benzene on cell viability. Buckwheat induced the stimulatory action of benzene on proliferation. Rooibos and vitex p...
Source: Environmental Science and Pollution Research International - Category: Environmental Health Authors: Tags: Environ Sci Pollut Res Int Source Type: research