Insights into oleaginous phenotype of the yeast Papiliotrema laurentii.

Insights into oleaginous phenotype of the yeast Papiliotrema laurentii. Fungal Genet Biol. 2020 Sep 07;:103456 Authors: Moreira Vieira N, Zandonade Ventorim R, Alexander de Moura Ferreira M, Barcelos de Souza G, Luís Menezes de Almeida E, Marcus Pereira Vidigal P, Nunes Nesi A, Gomes Fietto L, Batista da Silveira W Abstract Oleaginous yeasts have stood out due to their ability to accumulate oil, which can be used for fatty acid-derived biofuel production. Papiliotrema laurentii UFV-1 is capable of starting the lipid accumulation in the late exponential growth phase and achieves maximum lipid content at 48 h of growth; it is, therefore, interesting to study how its oleaginous phenotype is regulated. Herein, we provide for the first time insights into the regulation of this phenotype in P. laurentii UFV-1. We sequenced and assembled its genome, performed comparative genomic analyses and investigated its phylogenetic relationships with other yeasts. Gene expression and metabolomic analyses were carried out on the P. laurentii UFV-1 cultivated under a nitrogen-limiting condition. Our results indicated that the lipogenesis of P. laurentii might have taken place during evolution after the divergence of genera in the phylum Basidiomycota. Metabolomic data indicated the redirection of the carbon flux towards fatty acid synthesis in response to the nitrogen limitation. Furthermore, purine seems to be catabolized to recycle nitrogen and leuci...
Source: Fungal Genetics and Biology - Category: Biology Authors: Tags: Fungal Genet Biol Source Type: research
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