A Nosocomial Respiratory Infection Outbreak of Carbapenem-Resistant Escherichia coli ST131 With Multiple Transmissible blaKPC –2 Carrying Plasmids
In this study, 45 carbapenem-resistant E. coli strains were collected in a hospital. The resistance mechanisms, plasmid profiles, and genetic relatedness of these strains were determined. Phylogenetic relationships between these strains were assessed by molecular profiling and aligned with patient clinical details. The genetic context of blaKPC–2 was analyzed to trace the potential dissemination of blaKPC–2. The 45 carbapenem-resistant E. coli ST131 strains were closely related. Initially prevalent only in a single ward, ST131 subsequently spread to other ward, resulting in a respiratory infection outbreak of carbapenem-resistant E. coli ST131. Eight of the 30 patients died within 28 days of the first isolation of E. coli ST131. The blaKPC–2-positive plasmid profiles suggest that the carbapenem resistance was due to the acquisition by E. coli ST131 of transmissible plasmids pE0272_KPC and pE0171_KPC carrying blaKPC–2. Additionally, diverse multidrug resistance elements were transferred and rearranged between these plasmids mediated by IS26. Our research indicates that clinical attention should be paid to the importance of E. coli ST131 in respiratory infections and the spread of blaKPC-carrying E. coli ST131.
Conclusions: Our data has uncovered a prolonged clonal outbreak of ESBL S. sonnei that was likely first introduced by returned travellers and has subsequently been circulating locally in Australia. The emergence of a local outbreak of ESBL S. sonnei, with a multidrug-resistant profile, including reduced susceptibility to ciprofloxacin, represents a significant public health threat. PMID: 33020158 [PubMed - as supplied by publisher]
Authors: Ghita BT, Bennani L, Berrada S, Benboubker M, Bennani B Abstract Escherichia coli is related to foodborne disease and outbreaks worldwide. It mainly affects persons at high risk as newborns, infants, and individuals with impaired immune system in hospitals. Multidrug-resistant E. coli is currently spreading both in community and hospital settings. Our study aims to evaluate the presence of E. coli and the incidence of its antibiotic resistance in samples obtained from various cooked and raw foods (N = 300), food contact surfaces (N = 238), and food handlers (N = 40...
Conclusions Health communication and education efforts could be used to increase knowledge about shigellosis and shift perceptions about the severity of shigellosis among gay, bisexual, and other MSM. Additional work is needed to identify effective ways to promote shigellosis-related prevention behaviors among gay, bisexual, and other MSM.
This study provides an important baseline census of the standing pangenomic variation and drug resistance to aid the development of a statewide database for epidemiological studies and clinical decision making. Continued genomic surveillance will be necessary to accurately assess how the population of C. jejuni changes over the long term.
This study sheds light on the virulence and AMR potential of E. coli ST302 strains and informs AMR genomic surveillance.
In this study, we report a prolonged nosocomial outbreak of various NDM-1-producing Enterobacterales species due to clonal spread and cross-species exchange of plasmids and possibly transposons. Between July 2015 and September 2017, a total of 51 carbapenemase-positive isolates were collected from 38 patients and three environmental sources in a single German hospital. Combining molecular typing methods and whole genome sequencing, the metallo-β-lactamase gene blaNDM–1 was found to be present in 35 isolates of which seven additionally carried the carbapenemase gene blaKPC–2. Core genome MLST (cgMLST) revea...
Foodborne Pathogens and Disease, Ahead of Print.
(CNN) — When multidrug-resistant pathogens kept reappearing on the skin of premature babies in a German hospital’s neonatal intensive care unit, investigators were confused. Tests for contamination in the incubators, as well as among healthcare workers who came into contact with the preemies, were all coming up negative, according to a report on the case published Friday by the American Society for Microbiology. But tests showed the babies were continually being reinfected with Klebsiella oxytoca, a bacteria notorious for hospital-acquired infections such as pneumonia, urinary tract infection, soft tissue infec...
CONCLUSIONS: The high frequency of multidrug-resistant ESBL-producing isolates should alert the regional health authorities to take measures to reduce the risk of outbreaks caused by these types of bacteria in the community. PMID: 31529852 [PubMed - in process]
PMID: 31048673 [PubMed - in process]