Interferon- γ and IL-10 Release Assay for Patients with Ocular Toxoplasmosis.

Interferon-γ and IL-10 Release Assay for Patients with Ocular Toxoplasmosis. Am J Trop Med Hyg. 2020 Sep 08;: Authors: Rudzinski M, Pardini L, Bernstein M, Moré G, Khoury M, Duarte Szyszko SC, Argüelles C, Reina S, Oubiña JR Abstract Peripheral blood mononuclear cells (PBMCs) from patients with ocular toxoplasmosis were challenged with Toxoplasma gondii lysate antigen (TLA) in a cytokine release assay (CRA), run during the inactive period of the disease. Increased interferon gamma (IFN-γ) levels were detected after PBMC stimulation with either ME49 reference strain (P = 0.0015) or local TgCkAr-11-9 isolate (P = 0.0012), as compared with those recorded under basal conditions. TLA from TgCkAr11-9 isolate induced a higher release of IFN-γ than ME49 strain CRA from all tested patients (P = 0.02). The median value of IFN-γ release on TgCkAr-11-9 stimulation (26.03 pg/mL) allowed the classification of patients into high- or low-/non-IFN-γ releasers. Clinical correlations were established with both groups. The results obtained in this study suggest the need to include local strains when performing CRA with T. gondii TLA. PMID: 32901605 [PubMed - as supplied by publisher]
Source: The American Journal of Tropical Medicine and Hygiene - Category: Tropical Medicine Authors: Tags: Am J Trop Med Hyg Source Type: research