Assessment of cross-resistance potential to serial antibiotic treatments in antibiotic-resistant Salmonella Typhimurium

Publication date: Available online 8 September 2020Source: Microbial PathogenesisAuthor(s): Jirapat Dawan, Juhee Ahn
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research

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by Annica R. Stull-Lane, Kristen L. Lokken-Toyli, Vladimir E. Diaz-Ochoa, Gregory T. Walker, Stephanie A. Cevallos, Andromeda L. N. Winter, Ariel Del Hoyo Mu ñoz, Guiyan G. Yang, Eric M. Velazquez, Chun-Yi Wu, Renée M. Tsolis Disseminated disease from non-typhoidalSalmonella enterica strains results in>20% mortality globally. Barriers to effective treatment include emerging multidrug resistance, antibiotic treatment failure, and risk factors such as malnutrition and related micronutrient deficiencies. Individuals in sub-Saharan Africa are disproportionately affected by non-typhoidalS.enterica bloodstream ...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
In this study, we investigated whether a chronic exposure to a glyphosate-containing herbicide (GBH) results in resistance, a constitutive activation of the tolerance and stress response and cross-tolerance or cross-resistance to antibiotics. Of the ten farm animals-derived clinical isolates of Salmonella enterica subjected to experimental evolution in increasing concentrations of GBH, three isolates showed a stable resistance with mutations associated with the glyphosate target gene aroA and no fitness costs. The global quantitative proteomics analysis demonstrated activation of the cellular tolerance and stress response ...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research
Abstract Polymyxin B, used to treat infections caused by antibiotic-resistant Gram-negative bacteria, produces nephrotoxicity at its current dosage. We show that a combination of non-bactericidal concentration of this drug and lysophosphatidylcholine (LPC) potently inhibits growth of Salmonella and at least two other Gram-negative bacteria in vitro This combination makes bacterial membrane porous and causes degradation of the regulator of protein-folding, DnaK. Polymyxin B-LPC combination may be an effective and a safer regimen against drug resistant bacteria. PMID: 32988824 [PubMed - as supplied by publisher]
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research
This study aimed at detecting and profiling antibiotic resistance of bacterial contaminants in vended food and the environment. Seventy antibiotic-resistant bacterial isolates were isolated from fried fish, African sausages, roasted meat, smokies, samosa, chips (potato fries), vegetable salads, and soil samples collected from Embu Town and Kangaru Market in Embu County, Kenya. The antibiotic susceptibility test, morphological and biochemical characterization, antibiosis assay, polymerase chain reaction-based detection of antibiotic resistance genes, and sequencing of the 16S rRNA gene were done. Analysis of variance on all...
Source: International Journal of Microbiology - Category: Microbiology Tags: Int J Microbiol Source Type: research
Authors: Eve A, Aliero AA, Nalubiri D, Adeyemo RO, Akinola SA, Pius T, Nabaasa S, Nabukeera S, Alkali B, Ntulume I Abstract The current upsurge in resistance to conventional antibiotics, as well as high cost of orthodox medical treatment, called for the use of medicinal plants as an alternative therapy. This research was aimed at determining the antibacterial activity of Artocarpus heterophyllus seed extracts (Jackfruit as it is locally called) in the treatment of diarrhoea. Ethanolic and hexanolic seed crude extracts of the plant were screened for antidiarrhoeal activity against bacteria isolated from clinical sam...
Source: The Scientific World Journal - Category: Science Tags: ScientificWorldJournal Source Type: research
Abstract For decades, Salmonella Typhimurium and Salmonella Enteritidis have prevailed in several countries as agents of salmonellosis outbreaks. In Brazil, the largest exporter of poultry meat, relatively little attention has been paid to infrequent serovars. Here, we report the emergence and characterization of rare serovars isolated from food and related sources collected between 2014 and 2016 in Brazil. Twenty-two Salmonella enterica isolates were analyzed through the use of whole-genome sequencing (WGS) and clustered regularly interspaced short palindromic repeats (CRISPR) genotyping. These isolates were clas...
Source: Food Microbiology - Category: Food Science Authors: Tags: Food Microbiol Source Type: research
In this study, the preventive and therapeutic effects of phage PIZ SAE-01E2 against S. abortus equi in a mouse model of abortion was investigated. Phage PIZ SAE-01E2 was stable at different temperatures (4 ∼ 70 °C) and pH values (4 ∼ 10) and could lyse the majority of the tested Salmonella strains (25/28) of serotype O:4 and O:9. There is no lysogenic, toxin or antibiotic-resistant related gene in the genome of PIZ SAE-01E2. All of these characteristics indicated PIZ SAE-01E2 has potential for phage therapy. In in vivo experiments, 2 × 103 CFU/mouse of S. abortus equi ATCC 9842 was sufficient to lead to m...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research
In this study, a high flux screening based on nucleic acid isothermal amplification technique has been developed. For the 8 common pathogens, species-specific targets had been selected and analyzed for their unique specificity. After optimization, separate LAMP reaction assays had been bioprocessed and integrated into one systematic detection platform, including 8 strips (PCR tubes) and 96-well plates. Eight standard strains verified for the accuracy. Application of the established high flux screening platform was used for detection for 48 samples in 4 different 96-well plates, with 2 groups of 2 operators using double-bli...
Source: Bioprocess and Biosystems Engineering - Category: Biomedical Engineering Authors: Tags: Bioprocess Biosyst Eng Source Type: research
Abstract Restricting antibiotic use in food production animals is a target for reducing antimicrobial drug-resistant infections in humans. We used US surveillance data to estimate the probability of antibiotic-resistant nontyphoidal salmonellosis per meal made with beef during 2002-2010. Applying data for nontyphoidal Salmonella in raised-without-antibiotics cattle, we tested the effect of removing antibiotic use from all beef cattle production. We found an average of 1.2 (95% credible interval 0.6-4.2) antibiotic-resistant nontyphoidal salmonellosis cases per 1 million beef meals made with beef initially contamin...
Source: Emerging Infectious Diseases - Category: Infectious Diseases Authors: Tags: Emerg Infect Dis Source Type: research
CONCLUSIONS These results serve as an alert to the dangers of acquiring antibiotic-resistant bacteria from food and demonstrate the need to establish a surveillance system and institute measures bacte-rial control in food products.KEYWORDS Microbial drug resistance, bacteria, food, foodborne disease, Cuba. PMID: 32812898 [PubMed - in process]
Source: MEDICC Review - Category: International Medicine & Public Health Tags: MEDICC Rev Source Type: research
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